The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 07, 2008
Filed:
Jul. 25, 2006
Seung-goo Lee, Daejeon, KR;
Jae-jun Song, Daejeon, KR;
Jae-seok Ha, Daejeon, KR;
Young-mi Lee, Pohang-si, KR;
Jeong-min Lee, Daegu-si, KR;
Seung-Goo Lee, Daejeon, KR;
Jae-Jun Song, Daejeon, KR;
Jae-Seok Ha, Daejeon, KR;
Young-Mi Lee, Pohang-si, KR;
Jeong-Min Lee, Daegu-si, KR;
Korea Research Institute of Bioscience and Biotechnology, Daejeon, KR;
Abstract
A protein biosensor having increased signal intensity to sugar concentration and the use thereof. A fluorescent indicator protein is described, in which fluorescent proteins of different emission ranges are fused to both ends of a binding protein undergoing a conformational change due to binding of sugars, such that a change in the concentration of various sugars involved in intracellular metabolism, e.g., maltose, can be detected by measuring a change in emission intensities, caused by FRET (fluorescence resonance energy transfer). A method for detecting a change in the concentration of sugars using such fluorescent indicator protein is also described. The fluorescent indicator protein has excellent signal intensity enabling precision measurement of intracellular concentration of various sugars. In one implementation, high signal intensity fluorescent indicator proteins can be prepared from proteins otherwise used as biosensors, to enable them to be more widely useful.