The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 02, 2008
Filed:
Apr. 29, 2005
William H. Velander, Blacksburg, VA (US);
William N. Drohan, Springfield, VA (US);
Henryk Lubon, Rockville, MD (US);
Mary Ann H. Johnson, Legal Representative, Blacksburg, VA (US);
William H. Velander, Blacksburg, VA (US);
William N. Drohan, Springfield, VA (US);
Henryk Lubon, Rockville, MD (US);
Mary Ann H. Johnson, legal representative, Blacksburg, VA (US);
American Red Cross, Rockville, MD (US);
Virginia Tech Intellectual Properties, Inc., Blacksburg, VA (US);
Abstract
Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5'-untranslated and 3′-untranslated region, which is substituted with a 5-′ and 3′-end of the mouse whey acidic protein gene. In vitro cell cultures of cells explanted from the transgenic mammal of the invention and methods of producing Factor IX from such said culture and methods of treating hemophilia B are also described.