The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 12, 2008
Filed:
Feb. 16, 2005
Jonathan Beckwith, Cambridge, MA (US);
Fredrik Aslund, Stockholm, SE;
Paul H. Bessette, Camarillo, CA (US);
George Georgiou, Austin, TX (US);
Daniel Ritz, Everett, MA (US);
Jackie Eun-ah Lim, Shrewsbury, MA (US);
Jonathan Beckwith, Cambridge, MA (US);
Fredrik Aslund, Stockholm, SE;
Paul H. Bessette, Camarillo, CA (US);
George Georgiou, Austin, TX (US);
Daniel Ritz, Everett, MA (US);
Jackie Eun-ah Lim, Shrewsbury, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Board of Regents, University of Texas System, Austin, TX (US);
Abstract
The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.