The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 05, 2008
Filed:
Apr. 21, 2005
Jonas Anders, Darmstadt, DE;
Achim Schwämmle, Darmstadt, DE;
Sven Andrecht, Griesheim, DE;
Robertus Hendriks, Heidelberg, DE;
Anette Boerner, Darmstadt, DE;
Cora Rueter, Berlin, DE;
Jonas Anders, Darmstadt, DE;
Achim Schwämmle, Darmstadt, DE;
Sven Andrecht, Griesheim, DE;
Robertus Hendriks, Heidelberg, DE;
Anette Boerner, Darmstadt, DE;
Cora Rueter, Berlin, DE;
Merck Patent GmbH, Darmstadt, DE;
Abstract
The invention relates to a method and a kit for the isolation of phosphorylated peptides from complex mixtures using support materials having chelate ligands based on silica and alkaline elution buffers. In preferred embodiments, the method according to the invention enables the isolation of phosphopeptides from complex sample solutions with a reproducibly high yield and at the same time with high purity and it allows efficient ionisation and detection of the isolated phosphopeptides both by MALDI-TOF and also by ESI mass spectrometry without additional requisite method steps, such as, for example, chromatographic desalination of the sample.