The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 29, 2008
Filed:
Dec. 13, 2005
Melissa Hasenbank, Free Soil, MI (US);
Paul Yager, Seattle, WA (US);
Elain Fu, Seattle, WA (US);
Kjell Nelson, Seattle, WA (US);
Melissa Hasenbank, Free Soil, MI (US);
Paul Yager, Seattle, WA (US);
Elain Fu, Seattle, WA (US);
Kjell Nelson, Seattle, WA (US);
University of Washington UW Tech Transfer - Invention Licensing, Seattle, WA (US);
Abstract
The present invention provides methods and compositions for amplifying the detection signal in surface plasmon resonance (SPR)-based flow systems. The signal amplification methods comprise the use of well established marker systems that provide a precipitate. The marker systems include, for example, enzyme and nucleation systems. Enzymes suitable for use as a marker system include peroxidases and phosphatases. The amplification system is useful in any SPR-based detection system including microfluidic systems, e.g., 'lab on a chip' systems and the like. The methods can comprise any SPR-based assay format, including typical immunoassay formats. The immunoassay formats can include competitive and sandwich assays. Analyte capture agents can include antibodies, lectins, carbohydrates, polynucleotides, receptor proteins, and the like.