The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 22, 2008
Filed:
Jan. 15, 2004
Adelbert Bacher, Garching, DE;
Petra Adam, Kramsach, AT;
Wolfgang Eisenreich, Garching, DE;
Tobias Gräwert, Augsburg, DE;
Stefan Hecht, Bad Aibling, DE;
Felix Rohdich, Zolling, DE;
Ferdinand Zepeck, München, DE;
Adelbert Bacher, Garching, DE;
Petra Adam, Kramsach, AT;
Wolfgang Eisenreich, Garching, DE;
Tobias Gräwert, Augsburg, DE;
Stefan Hecht, Bad Aibling, DE;
Felix Rohdich, Zolling, DE;
Ferdinand Zepeck, München, DE;
Adelbert Bacher, Garching, DE;
Abstract
Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: (a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphnte reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; (b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P); (c) repeating step (a) in the presence of the sample to be tested; (d) repeating step (b) with the reaction mixture defined in step (c); (e) comparing the results of steps (b) and (d).