The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 2008
Filed:
Jan. 20, 2004
Yoshifumi Yanagita, Akashi, JP;
Noriko Ogawa, Kobe, JP;
Yasuyoshi Ueda, Himeji, JP;
Fumio Osakada, Okayama, JP;
Keiji Matsumoto, Nishinomiya, JP;
Yoshifumi Yanagita, Akashi, JP;
Noriko Ogawa, Kobe, JP;
Yasuyoshi Ueda, Himeji, JP;
Fumio Osakada, Okayama, JP;
Keiji Matsumoto, Nishinomiya, JP;
Kaneka Corporation, Osaka-shi, JP;
Abstract
The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles. The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.