The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 2008
Filed:
May. 06, 2004
Satoru Oonaka, Tokyo, JP;
Toshinori Hayashi, Kanagawa-ken, JP;
Satoru Oonaka, Tokyo, JP;
Toshinori Hayashi, Kanagawa-ken, JP;
Tosoh Corporation, Shunan-shi, JP;
Abstract
A method of detecting micrometastasis of tumor cells in a sample obtained from a region of a subject other than the primary focus of the tumor by using a first primer complementary to part of a specific sequence in the RNA from tumor cells and a second primer homologous to part of the specific sequence (either of which additionally has a promoter sequence for an RNA polymerase at the 5' end), which comprises (1) synthesizing a cDNA by the action of an enzyme having RNA-dependent DNA polymerase activity by using the specific sequence as a template, (2) degrading the RNA strand in the RNA-DNA double strand by an enzyme having a ribonuclease H activity (to give a single-stranded DNA), (3) forming a double-stranded DNA having a promoter sequence which can be transcribed into an RNA homologous or complementary to the specific sequence by using the single-stranded DNA as a template by the action of an enzyme having DNA-dependent DNA polymerase activity, and (4) transcribing the double-stranded DNA into an RNA transcript (which acts as a template in the subsequent cDNA synthesis in the reaction (1)) by the action of an enzyme having RNA polymerase activity and detecting the mRNA.