The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 01, 2008
Filed:
Feb. 05, 2004
Shiv Kumar, Belle Mead, NJ (US);
Mark Mcdougall, Arroyo Grande, CA (US);
Anup Sood, Flemington, NJ (US);
John Nelson, Hillsborough, NJ (US);
Carl Fuller, Berkeley Heights, NJ (US);
John Macklin, Wenonah, NJ (US);
Paul Mitsis, Trenton, NJ (US);
Shiv Kumar, Belle Mead, NJ (US);
Mark McDougall, Arroyo Grande, CA (US);
Anup Sood, Flemington, NJ (US);
John Nelson, Hillsborough, NJ (US);
Carl Fuller, Berkeley Heights, NJ (US);
John Macklin, Wenonah, NJ (US);
Paul Mitsis, Trenton, NJ (US);
GE Healthcare Bio-Sciences Corp., Piscataway, NJ (US);
Abstract
The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.