The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 24, 2008

Filed:

Jun. 11, 2001
Applicants:

Kazuhiro Nakashima, Miki, JP;

Tsuneyoshi Torii, Kobe, JP;

Hiroshi Tsuchiya, Kobe, JP;

Shinya Uchida, Kobe, JP;

Aya Konishi, Kobe, JP;

Kunio Tanaka, Kakogawa, JP;

Inventors:

Kazuhiro Nakashima, Miki, JP;

Tsuneyoshi Torii, Kobe, JP;

Hiroshi Tsuchiya, Kobe, JP;

Shinya Uchida, Kobe, JP;

Aya Konishi, Kobe, JP;

Kunio Tanaka, Kakogawa, JP;

Assignee:

Sysmex Corporation, Kobe-Shi, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/546 (2006.01);
U.S. Cl.
CPC ...
Abstract

An immunoassay comprises the steps of: (a) mixing a whole blood sample with sensitized insoluble carrier particles smaller than erythrocytes to cause an immune agglutination reaction; (b) introducing the resulting immune agglutination reaction mixture including agglutinated particles and unagglutinated particles to a flow cell, irradiating the particles passing through the flow cell with laser light, and detecting scattered lights generated thereby; (c) setting a threshold value for distinguishing unagglutinated particles from agglutinated particles and a threshold value for distinguishing the agglutinated particles from blood cells with regard to intensity of the scattered light; and (d) distinguishing and counting the unagglutinated particles, the agglutinated particles and the blood cells from the scattered lights detected in the step (b), in reference to the threshold values set in the step (c).


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