The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 10, 2008

Filed:

Oct. 31, 2003
Applicant:

Karla M. Robotti, Mountain View, CA (US);

Inventor:

Karla M. Robotti, Mountain View, CA (US);

Assignee:

Agilent Technologies, Inc., Santa Clara, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/00 (2006.01); C12P 21/06 (2006.01);
U.S. Cl.
CPC ...
Abstract

A method useful in the analysis of glycosylated proteins, in which a mixture containing glycosylated proteins and unglycosylated proteins is contacted with a resin that includes a nucleophile bound to a solid support via a linker. The contacting is performed under conditions sufficient to result in removal of the glycosyl group from the glycosylated proteins and to concomitantly result in the deglycosylated proteins covalently bound to the solid support. The deglycosylated proteins bound to the solid support may be rinsed to remove proteins that are not covalently bound to the solid support. The deglycosylated proteins are released from the solid support and may be subjected to further purification and/or analysis.


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