The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 29, 2008
Filed:
Mar. 05, 2004
Satoshi Hashiguchi, Kyoto, JP;
Ken Inose, Kyoto, JP;
Satoshi Hashiguchi, Kyoto, JP;
Ken Inose, Kyoto, JP;
Arkray, Inc., Kyoto-shi, Kyoto, JP;
Abstract
In a method for amplifying a DNA, comprising performing PCR using a sense primer and an antisense primer, PCR is performed in the presence of an additional sense primer comprising the sense primer and an oligodeoxyribonucleotide having a first additional sequence and ligated to the 5' end of the sense primer and an additional antisense primer comprising the antisense primer and an oligodeoxyribonucleotide having a second additional sequence complementary to the first additional sequence and ligated to the 5′ end of the antisense primer; a Tm value of the additional sequences is lower than Tm values of the sense primer and the antisense primer; and annealing temperature in PCR is initially set to be a temperature at which the additional sequences do not anneal and changed in the course of PCR to a temperature at which the additional sequences anneal to each other.