The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 29, 2008
Filed:
May. 02, 2005
Mark R. Andersen, Carlsbad, CA (US);
Jer-kang Chen, Palo Alto, CA (US);
Michael W. Hunkapiller, San Carlos, CA (US);
Steven M. Menchen, Fremont, CA (US);
Mark R. Andersen, Carlsbad, CA (US);
Jer-Kang Chen, Palo Alto, CA (US);
Michael W. Hunkapiller, San Carlos, CA (US);
Steven M. Menchen, Fremont, CA (US);
Applera Corporation, Foster City, CA (US);
Abstract
Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.