The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 22, 2008
Filed:
Dec. 28, 2004
Helen Fillmore, Richmond, VA (US);
Shelley Hoover, Richmond, VA (US);
William C. Broaddus, Midlothian, VA (US);
George Gillies, Charlottesville, VA (US);
Helen Fillmore, Richmond, VA (US);
Shelley Hoover, Richmond, VA (US);
William C. Broaddus, Midlothian, VA (US);
George Gillies, Charlottesville, VA (US);
Creative Science Solutions, Inc., Richmond, VA (US);
Abstract
Cells are generated from skin biopsies for use in cell implantation by identifying a source of skin cells that have a surface concentration of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; taking a sample of tissue from the surface area; mechanically disaggregating the tissue samples; collecting the disaggregated cells; washing the disaggregated cells; filtering the washed disaggregated cells; providing a cell suspension with filtered and washed keritinocytes and/or melanocytes; and suspending the cell suspension in a medium. In greater particularity, the method may comprise a) identifying a source of skin cells that have a surface concentration of at least 400 cells per square millimeter of surface area of at least one cell selected from the group consisting of keritinocytes and/or melanocytes; b) taking a sample of those skin cells and culturing them to enrich for cells selected from the group consisting of keritinocytes and melanocytes to form cultured cells for transplantation purposes c) concentrating the cells selected from the group consisting of keritinocytes and/or melanocytes from the cultured mass of cells; and d) differentiating the concentrated cells selected from the group consisting of keritinocytes an/or melanocytes.