The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 15, 2008
Filed:
Oct. 19, 2001
Astrid Vrang, Helsinge, DK;
Søren Michael Madsen, Copenhagen, DK;
Lars Bredmose, Copenhagen, DK;
Peter Ravn, Naerum, DK;
Jose Arnau, Hellerup, DK;
Mads Gronvald Johnsen, Frederiksberg, DK;
Anne Cathrine Steenberg, Horsholm, DK;
Hans Israelsen, Allerod, DK;
Astrid Vrang, Helsinge, DK;
Søren Michael Madsen, Copenhagen, DK;
Lars Bredmose, Copenhagen, DK;
Peter Ravn, Naerum, DK;
Jose Arnau, Hellerup, DK;
Mads Gronvald Johnsen, Frederiksberg, DK;
Anne Cathrine Steenberg, Horsholm, DK;
Hans Israelsen, Allerod, DK;
Bioneer A/S, Horsholm, DK;
Abstract
The invention provides a fed-batch or continuous process for producing heterologous peptides, polypeptides or proteins in lactic acid bacteria, comprising the steps of (i) constructing a recombinant lactic acid bacterium comprising a nucleotide sequence coding for the heterologous peptide, polypeptide or protein, and appropriate regulatory nucleotide sequences such as regulatable promoters and signal peptides to control the expression of the coding sequence and the secretion of gene product, (ii) cultivating the recombinant bacterium under or continuous cultivation conditions to express the gene, and (iii) harvesting the recombinant bacterium or the gene product. Preferably, the cultivation medium is a chemically defined or synthetic medium optionally supplemented with yeast extract.