The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 25, 2008
Filed:
Oct. 20, 2004
Eva Hoess, Munich, DE;
Thomas Meier, Munich, DE;
Gabriele Pestlin, Munich, DE;
Friedrich Popp, Penzberg, DE;
Klaus Reichert, Wilheim, DE;
Rainer Schmuck, Benediktbeuern, DE;
Bernd Schneidinger, Hohenschaeftlarn/Neufahrn, DE;
Christoph Seidel, Weilheim, DE;
Wilhelm Tischer, Peissenberg, DE;
Eva Hoess, Munich, DE;
Thomas Meier, Munich, DE;
Gabriele Pestlin, Munich, DE;
Friedrich Popp, Penzberg, DE;
Klaus Reichert, Wilheim, DE;
Rainer Schmuck, Benediktbeuern, DE;
Bernd Schneidinger, Hohenschaeftlarn/Neufahrn, DE;
Christoph Seidel, Weilheim, DE;
Wilhelm Tischer, Peissenberg, DE;
Hoffmann-La Roche Inc., Nutley, NJ (US);
Abstract
A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.