The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 18, 2008

Filed:

Aug. 29, 2003
Applicants:

Eun Jung Lee, Gyeonggi-do, KR;

Hyun Seok Kim, Seoul, KR;

Jong Sang Chung, Seoul, KR;

Ki Wan Kim, Seoul, KR;

Yeon Hyang Kim, Seoul, KR;

Hyune Soo Lee, Seoul, KR;

Hyung Kon Koh, Seoul, KR;

Myung Suk OH, Gyeonggi-do, KR;

Inventors:

Eun Jung Lee, Gyeonggi-do, KR;

Hyun Seok Kim, Seoul, KR;

Jong Sang Chung, Seoul, KR;

Ki Wan Kim, Seoul, KR;

Yeon Hyang Kim, Seoul, KR;

Hyune Soo Lee, Seoul, KR;

Hyung Kon Koh, Seoul, KR;

Myung Suk Oh, Gyeonggi-do, KR;

Assignee:

CJ Corp, Seoul, KR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/27 (2006.01); C12N 15/66 (2006.01); C07K 14/535 (2006.01); A61K 38/18 (2006.01);
U.S. Cl.
CPC ...
Abstract

Disclosed are human G-CSF isoforms having a modified amino acid sequence containing a glycosylation sequence Asn-X-Ser/Thr (N-X-S/T) at one or more specific amino acid positions according to the present invention, genes encoding the human G-CSF isoforms, and expression vectors carrying the genes, eukaryotic cells transformed or transfected with the expression vectors. Also, the present invention discloses a method of preparing a glycosylated human G-CSF isoform, comprising the steps of culturing the transformant or transfectant and isolating a glycosylated human G-CSF isoform from the culture supernatant or cell lysates, a human G-CSF isoform prepared by the method, and a pharmaceutical composition comprising the human G-CSF isoform.


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