The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 12, 2008
Filed:
Oct. 12, 2004
Michael Kirchgesser, Taufkirchen, DE;
Frank Bergmann, Iffeldorf, DE;
Thomas Walter, Penzberg, DE;
Kurt Weindel, Wielenbach-Hardt, DE;
Ralf Zielenski, Benediktbeuern, DE;
Emad Sarofim, Hagendorn, CH;
Michael Kirchgesser, Taufkirchen, DE;
Frank Bergmann, Iffeldorf, DE;
Thomas Walter, Penzberg, DE;
Kurt Weindel, Wielenbach-Hardt, DE;
Ralf Zielenski, Benediktbeuern, DE;
Emad Sarofim, Hagendorn, CH;
Roche Diagnostics Operations, Inc., Indianapolis, IN (US);
Abstract
A method for purification of a nucleic acid comprising the steps of (a) adsorbing on a substrate the nucleic acid from a composition containing (i) an aqueous buffer, (ii) salts in a high concentration, (iii) a water-miscible, non-acidic organic compound, and (iv) the nucleic acid; (b) optionally washing with a washing solution the substrate with the adsorbed nucleic acid; (c) contacting the substrate with the adsorbed nucleic acid with a solution containing salts in a lower concentration compared to the composition of step (a), thereby desorbing the nucleic acid from the substrate; (d) separating the solution with the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid; and optionally (e) precipitating the desorbed nucleic acid from the solution of step (d) and isolating the precipitated nucleic acid, thereby further purifying the nucleic acid.