The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 22, 2008

Filed:

Jul. 06, 2004
Applicants:

Philippe Talaga, Sainte Consorce, FR;

Patricia Sepulcri, Marcy l'Etoile, FR;

Sandrine Vialle-blanc, Tassin la Demi Lune, FR;

Inventors:

Philippe Talaga, Sainte Consorce, FR;

Patricia Sepulcri, Marcy l'Etoile, FR;

Sandrine Vialle-Blanc, Tassin la Demi Lune, FR;

Assignee:

Aventis Pasteur SA, Lyons, FR;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/569 (2006.01); C07K 1/12 (2006.01);
U.S. Cl.
CPC ...
Abstract

The invention relates to a method for assaying the teichoic acids present, most commonly in residual form, in a preparation of Gram+ bacterial antigens. This method requires, first of all, a controlled hydrolysis with hydrofluoric acid at a temperature of less than or equal to 40° C., in order to release the oligosaccharides specific for teichoic acids. The assaying of the specific oligosaccharides can then be carried out by various techniques, in particular by high performance chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The method according to the invention can in particular be used to assay the residual amounts of teichoic acids present in preparations containing capsular polysaccharides ofwhich may be useful as vaccines.


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