The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 15, 2008
Filed:
Mar. 07, 2003
Ho-sun Son, Seoul, KR;
Do-hui Kim, Busan, KR;
Neon-c Jung, Seoul, KR;
Eun-wook Choi, Seoul, KR;
Dong-seung Seen, Seoul, KR;
Min-sung Kim, Gyeonggi-do, KR;
Yong-weon Yi, Daejeon, KR;
Kyung-jin Kim, Seoul, KR;
Ho-Sun Son, Seoul, KR;
Do-Hui Kim, Busan, KR;
Neon-C Jung, Seoul, KR;
Eun-Wook Choi, Seoul, KR;
Dong-Seung Seen, Seoul, KR;
Min-Sung Kim, Gyeonggi-do, KR;
Yong-Weon Yi, Daejeon, KR;
Kyung-Jin Kim, Seoul, KR;
Neurogenex Co., Ltd., Seoul, KR;
Abstract
Disclosed are the methods for producing recombinant viruses using site-specific recombination in vitro. In the present invention, circular viral genomic DNAs are digested with restriction enzymes to generate a linear form viral genomic DNAs flanked by site-specific recombination sites, and then are subjected to site-specific recombination with the desired genomic materials flanked by site-specific recombination sites in vitro. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the desired recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system for generating and screening hundreds or thousands of recombinant viruses.