The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 06, 2007
Filed:
Dec. 10, 2002
Marc L. Pusey, Huntsville, AL (US);
Krishnan K. Chittur, Huntsville, AL (US);
Jeffrey J. Dowell, Laceys Spring, AL (US);
Joseph D. NG, Huntsville, AL (US);
Marc L. Pusey, Huntsville, AL (US);
Krishnan K. Chittur, Huntsville, AL (US);
Jeffrey J. Dowell, Laceys Spring, AL (US);
Joseph D. Ng, Huntsville, AL (US);
University of Alabama at Huntsville, Huntsville, AL (US);
Abstract
A nucleic acid detector and a method of using the detector are disclosed for detecting the presence of target nucleic acid sequences within a sample. The nucleic acid detector comprises a substrate-bound, hairpin-shaped nucleic acid captor in conjunction with a labeled universal nucleic acid detector probe. The captor has a segment that is complementary to at least a portion of the target nucleic acid and is denatured and hybridized to the target under test conditions. Hybridization of the captor to the target maintains the captor in an open conformation which exposes an end portion of the captor to the universal detector probe. The detector probe is able to hybridize with the exposed end portion of the captor if the captor has hybridized with a target. The labeled detector probe is detectable by external detection methods. Detector probes having identical universal detector probe sequences may be used to identify the presence of multiple targets having various target sequences within a sample. An assay embodying the detector and method is also contemplated.