The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 30, 2007
Filed:
Jul. 18, 2003
Applicants:
Willem P. C. Stemmer, Los Gatos, CA (US);
Andreas Crameri, Los Altos, CA (US);
Inventors:
Willem P. C. Stemmer, Los Gatos, CA (US);
Andreas Crameri, Los Altos, CA (US);
Assignee:
Maxygen, Inc., Redwood City, CA (US);
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 21/02 (2006.01); C12P 19/34 (2006.01); C12P 21/08 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
Abstract
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.