The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 02, 2007
Filed:
Jun. 02, 2004
Max N. Moore, Encinitas, CA (US);
Mark Andrade, Vista, CA (US);
Recaldo Carty, Carlsbad, CA (US);
Anthony Scozzari, Vista, CA (US);
Achim Krotz, San Diego, CA (US);
Max N. Moore, Encinitas, CA (US);
Mark Andrade, Vista, CA (US);
Recaldo Carty, Carlsbad, CA (US);
Anthony Scozzari, Vista, CA (US);
Achim Krotz, San Diego, CA (US);
Isis Pharmaceuticals, Inc., Carlsbad, CA (US);
Abstract
The invention provides for methods of manufacturing an oligonucleotide comprising a pentavalent phosphate triester. In particular, the method comprises providing a 5' blocked-nucleoside, deblocking the 5′ blocked-nucleoside to form a 5′ OH-nucleoside, coupling the 5′ OH-nucleoside with a phosphoramidite to form and oligonucleotide comprising a trivalent phosphite triester; and oxidizing the oligonucleotide comprising a trivalent phosphite triester to the oligonucleotide comprising a pentavalent phosphate triester. In some embodiments, the wash between any of the steps above is with at least one solvent wash comprising a toluene.
Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile