The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 02, 2007
Filed:
Dec. 12, 1997
Gerlinde Lenzen, Paris, FR;
Arthur Donny Strosberg, Paris, FR;
Toshinari Sugasawa, Osaka, JP;
Shigeaki Morooka, Hyogo, JP;
Gerlinde Lenzen, Paris, FR;
Arthur Donny Strosberg, Paris, FR;
Toshinari Sugasawa, Osaka, JP;
Shigeaki Morooka, Hyogo, JP;
Dainippon Sumitomo Pharma Co., Ltd., Osaka, JP;
Abstract
An isolated and substantially pure mammal polypeptide different from known adrenergic, serotonine and dopamine receptors, existing at least on mammalian muscle and eosinophils membranes, for instance in rat, guinea pig and humans. The invention also relates to plasmids containing the genes coding for said polypeptide, to host cells transformed by genes coding for the above mentioned polypeptide, to nucleotide probes capable of hybridizing with the genes coding for the above mentioned polypeptide, and to polyclonal and monoclonal antibodies directed against the above mentioned polypeptide. Said polypeptide is characterized in that it contains sites such that when said sites are exposed at the surface of a cell, they are able of binding iodocyanopindolol (ICYP) under blockage of α, β1, β2, β3-AR, serotonine 5-HTand serotonine 5-HTreceptors, said binding being saturable, reversible, able to be displaced by a β-adrenergic receptor agonist SM-11044 with stereoselectivity but not by isoproterenol, norepi-nephrine, epinephrine, serotonine, dopamine or BRL-37344, and not being blocked by propranolol, said polypeptide (1) having an apparent molecular weight of about 30-40 kDa when labeled withI-iodocyanopindolol after photoaffinity labeling and separation by electrophoresis and an apparent molecular weight of about 60-80 kDa in Western blot, and (2) generating a fragment having the following formula DPXFFQHRIHXFSIFNXby acidic cleavage, wherein Xrepresents S (SEQ ID NO.5) or X (SEQ ID NO.6), Xrepresents V (SEQ ID NO.6) or W (SEQ ID NO.5) and Xrepresents S (SEQ ID NO.5) or H (SEQ ID NO.6), said polypeptide being present at least on muscles and eosinophils membranes and being a non-adrenergic receptor.