The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 04, 2007
Filed:
Mar. 18, 2003
Oren Bogin, Moshav Ganei Yohanan, IL;
Avner Yayon, Moshav Sitria, IL;
Moshe Peretz, Moshav Beit Gamliel, IL;
Yigal Burstein, Rehovot, IL;
Oren Bogin, Moshav Ganei Yohanan, IL;
Avner Yayon, Moshav Sitria, IL;
Moshe Peretz, Moshav Beit Gamliel, IL;
Yigal Burstein, Rehovot, IL;
ProChon Biotech Ltd., Rehovot, IL;
Yeda Research and Development Co., Ltd., Rehovot, IL;
Abstract
The present invention discloses the isolation and use of a specific bacterial promoter region suitable for use in constructs for the high level production of heterologous proteins. This promoter is derived from the bacterial gene encoding for alcohol dehydrogenase, in particular the alcohol dehydrogenase genes isolated from the thermophilic bacterial strainand the mesophilic bacterial strain. It is now disclosed that using either the intact promoter region or certain specific fragments consisting of at least a 88 bp DNA sequence in the upstream untranslated region of the bacterial alcohol dehydrogenase gene, operatively linked to the nucleic acid sequences encoding a heterologous protein, and insertion into a DNA plasmid or any other suitable vector system, heterologous genes can be expressed in high levels in host cells. Heterologous proteins or peptides can be expressed constitutively at high levels. The proteins are obtained in their active folded form.