The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 14, 2007
Filed:
Sep. 28, 2000
Kei Tashiro, Kyoto, JP;
Tasuku Honjo, Kyoto, JP;
Masaya Ikegawa, Kyoto, JP;
Kazuko Matsumoto, Setagaya, JP;
Kei Tashiro, Kyoto, JP;
Tasuku Honjo, Kyoto, JP;
Masaya Ikegawa, Kyoto, JP;
Kazuko Matsumoto, Setagaya, JP;
Japan Science and Technology Corporation, Saitama, JP;
Abstract
A method for detecting a cytokine in a biological fluid sample with a high sensitivity is provided. A time-resolved fluoroimmunoassay (TR-FIA) method including a step of forming on a solid phase a composite in which a cytokine is captured and which includes a fluorescent structural portion which has been complexed with a lanthanoid metal ion, and measuring fluorescence of the fluorescent structural portion. The composite is formed of a structure in which (a) a first antibody including a portion bound to a solid phase and a region bindable to a cytokine; (b) the cytokine; (c) a second antibody including a region bindable to the cytokine and a portion to which biotin is bound; (d) a conjugate including streptoavidin or avidin and a fluorescent structural portion capable of being complexed with a lanthanoid metal ion; and (e) the lanthanoid metal ion are bound. The fluorescent structural portion is represented by General Formula (I): R—Ar—C(═O)—CH—C(═O)—CF—X.