The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 07, 2007
Filed:
Sep. 30, 2003
Yoshimi Kikuchi, Kawasaki, JP;
Masayo Date, Kawasaki, JP;
Yukiko Umezawa, Kawasaki, JP;
Keiichi Yokoyama, Kawasaki, JP;
Haruo Heima, Kawasaki, JP;
Hiroshi Matsui, Kawasaki, JP;
Yoshimi Kikuchi, Kawasaki, JP;
Masayo Date, Kawasaki, JP;
Yukiko Umezawa, Kawasaki, JP;
Keiichi Yokoyama, Kawasaki, JP;
Haruo Heima, Kawasaki, JP;
Hiroshi Matsui, Kawasaki, JP;
Ajinomoto Co., Inc., Tokyo, JP;
Abstract
The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild typeATCC 13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.