The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 31, 2007
Filed:
Mar. 07, 2005
Peter Ruhdal Jensen, Gentofte, DK;
Jacky Leendert Snoep, Amsterdam, NL;
Hans Victor Westerhoff, Amsterdam, NL;
Peter Ruhdal Jensen, Gentofte, DK;
Jacky Leendert Snoep, Amsterdam, NL;
Hans Victor Westerhoff, Amsterdam, NL;
Other;
Abstract
The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F) of the membrane bound (FFtype) HATPase or a portion of Fexhibiting ATPase activity. The organism from which the FATPase or portions thereof is derived, or in which the FATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding For a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the Fsubunit β or a portion thereof and various combinations of the gene or portion with the genes encoding the other Fsubunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing level of ATPase expression at which the conversion rate is optimized.