The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 17, 2007

Filed:

Aug. 01, 2002
Applicants:

Takeshi Koizumi, Chiba, JP;

Satoshi Yamamoto, Chiba, JP;

Takeshi Itoh, Tokyo, JP;

Hiroshi Nakagawa, Tokyo, JP;

Inventors:

Takeshi Koizumi, Chiba, JP;

Satoshi Yamamoto, Chiba, JP;

Takeshi Itoh, Tokyo, JP;

Hiroshi Nakagawa, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C07H 21/02 (2006.01); C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
Abstract

To prepare high-performance specific gene-amplification primers for detecting, quantitatively determining or identifyinghaving low possibility of mis-identification and practically sufficient amplification efficiency and amplification specificity. We have determined the nucleotide sequences of rpoD genes encoding RNA polymerase σ70 factor of type strains of the genusand of stock strains of(strains containing and those not containing toxin gene); elucidated the phylogenetic relation, and identified nucleotides which are characteristic inthereby enabling the design of probes containing them and having high specificity, and primers for gene amplification having high specificity and excellent amplification efficiency.


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