The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 17, 2007
Filed:
Nov. 15, 2004
Lázaro Hiram Betancourt Núñez, Ciudad de la Habana, CU;
Jorge Fernández DE Cossio Dorta-duque, Ciudad de la Habana, CU;
Vladimir Armando Besada Pérez, Ciudad de la Habana, CU;
Jeovanis Gil Valdés, Ciudad de la Habana, CU;
Luis Javier González López, Ciudad de la Habana, CU;
Gabriel Ramón Padrón Palomares, Ciudad de la Habana, CU;
Rolando Pajón Feyt, La Habana, CU;
Félix Modesto Álvarez Gil, Ciudad de la Habana, CU;
Lázaro Hiram Betancourt Núñez, Ciudad de la Habana, CU;
Jorge Fernández de Cossio Dorta-Duque, Ciudad de la Habana, CU;
Vladimir Armando Besada Pérez, Ciudad de la Habana, CU;
Jeovanis Gil Valdés, Ciudad de la Habana, CU;
Luis Javier González López, Ciudad de la Habana, CU;
Gabriel Ramón Padrón Palomares, Ciudad de la Habana, CU;
Rolando Pajón Feyt, La Habana, CU;
Félix Modesto Álvarez Gil, Ciudad de la Habana, CU;
Centro de Ingenieria Genetica y Biotecnologia, C. Habana, CU;
Abstract
The present invention describes a method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixture. The method comprises the selective isolation from every protein of those peptides that neither contain arginine nor histidine (NHNR peptides), and the determination of the relative concentrations of one or several proteins in different samples from the ratio between the areas of the estimated theoretical spectra for the NHNR peptides labeled with different isotopes in each sample. The determination of the relative concentration of proteins is valid for any type of isotopic label of the NHNR peptides. The method avoids the separation and purification of the proteins present in a complex mixture, and the analysis of all peptides generated from the enzymatic digest of the samples. The method is applicable to the identification of proteins with vacunal, therapeutic and diagnostic aims.