The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 10, 2007
Filed:
Jul. 15, 2002
Theodorus Sonke, Guttecoven, NL;
Renate Francisca Tandler, Buchten, NL;
Cornelis Gerrit Nijs Korevaar, Katwijk, NL;
Friso Bernard Jan Van Assema, Geleen, NL;
Rudolf Van Der Pol, Schiedam, NL;
Theodorus Sonke, Guttecoven, NL;
Renate Francisca Tandler, Buchten, NL;
Cornelis Gerrit Nijs Korevaar, Katwijk, NL;
Friso Bernard Jan Van Assema, Geleen, NL;
Rudolf Van Der Pol, Schiedam, NL;
DSM IP Assets B.V., TE Heerlen, NL;
Abstract
The invention relates to nucleic acid sequence encoding enantioselective amides with an amino acid sequence of SEQ ID NO: 2. The invention also relates to a process for fermentation, comprising a batch and a feed phase, of a microorganism in a fermentation medium, wherein the microorganism expresses a nucleic acid according to the invention and wherein between 0.5 and 50 mg of Zn/ml of fermentation medium is fed during the fermentation. The invention also relates to a process for the preparation of enatiomerically enriched carboxylic acid and/or an enatiomerically enriched carboxylic acid amide, in which a mixture of corresponding D- and L-carboxylic acid amides is contacted with a expression product according to the invention in the presence of 0.01 mM–100 mM Zn, whereby one of the enantiomers of the carboxylic acid amide is enatiomerically hydrolysed to from corresponding enatiomerically enriched carboxylic acid, while the other enantiomer of the carboxylic acid amide remains unchanged.