The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 10, 2007

Filed:

May. 10, 2002
Applicants:

Seung-kwon Noh, Seoul, KR;

Myung-kuk Kim, Seoul, KR;

Won-tae Yoon, Seoul, KR;

Kyung-moon Park, Gyeonggi-do, KR;

Sang-ok Park, Seoul, KR;

Inventors:

Seung-Kwon Noh, Seoul, KR;

Myung-Kuk Kim, Seoul, KR;

Won-Tae Yoon, Seoul, KR;

Kyung-Moon Park, Gyeonggi-do, KR;

Sang-Ok Park, Seoul, KR;

Assignee:

Eugene Science Inc., Seoul, KR;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 33/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention relates to a method for preparation of androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD). More particularly, the present invention relates to a method for preparing AD/ADD, comprising the steps of: (a) heating sterols and emulsifier respectively, to dissolve completely and mixing to prepare a mixture; (b) placing the mixture in a water bath at 70˜90° C. and stirring to obtain emulsified sterols; and (c) adding the emulsified sterols to culture media of microorganism as a component. And the present invention relates to a method for preparing AD/ADD, comprising the addition of cyclodextrin-sterol complexes extracted from milk to culture media of microorganism as a component. The emulsified sterols and cyclodextrin-sterol complexes adopted in the present invention can be easily prepared and be effectively used for obtaining AD/ADD at a high yield.


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