The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 05, 2007
Filed:
Dec. 10, 2002
Kaio Kitazato, Ibaraki, JP;
Tsugumine Shu, Ibaraki, JP;
Hidekazu Kuma, Ibaraki, JP;
Yasuji Ueda, Ibaraki, JP;
Makoto Asakawa, Osaka, JP;
Mamoru Hasegawa, Ibaraki, JP;
Akihiro Iida, Ibaraki, JP;
Fumino Tokito, Ibaraki, JP;
Takahiro Hirata, Ibaraki, JP;
Tsuyoshi Tokusumi, Ibaraki, JP;
Makoto Inoue, Ibaraki, JP;
Yumiko Tokusumi, Ibaraki, JP;
Kaio Kitazato, Ibaraki, JP;
Tsugumine Shu, Ibaraki, JP;
Hidekazu Kuma, Ibaraki, JP;
Yasuji Ueda, Ibaraki, JP;
Makoto Asakawa, Osaka, JP;
Mamoru Hasegawa, Ibaraki, JP;
Akihiro Iida, Ibaraki, JP;
Fumino Tokito, Ibaraki, JP;
Takahiro Hirata, Ibaraki, JP;
Tsuyoshi Tokusumi, Ibaraki, JP;
Makoto Inoue, Ibaraki, JP;
Yumiko Tokusumi, Ibaraki, JP;
DNAVEC Research Inc., Tsukuba-Shi, Ibaraki, JP;
Abstract
F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.