The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 29, 2007
Filed:
Apr. 27, 2002
Min Zhang, Lakewood, CO (US);
Yat-chen Chou, Golden, CO (US);
William Howe, Golden, CO (US);
Christine Eddy, Golden, CO (US);
Kent Evans, Littleton, CO (US);
Ali Mohagheghi, Northglenn, CO (US);
Min Zhang, Lakewood, CO (US);
Yat-Chen Chou, Golden, CO (US);
William Howe, Golden, CO (US);
Christine Eddy, Golden, CO (US);
Kent Evans, Littleton, CO (US);
Ali Mohagheghi, Northglenn, CO (US);
Midwest Research Institute, Kansas City, MO (US);
Abstract
Disclosed in the present invention is aintegrant and derivatives of these integrants that posses the ability to ferment pentose into ethanol. The genetic sequences encoding for the pentose-fermenting enzymes are integrated into thein a two-integration event of homologous recombination and transposition. Each operon includes more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequences encoding xylose isomerase, xylulokinase, transketolase and transketolase. Theintegrants are highly stable, and retain activity for producing the pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetate per liter media. These stably integrated sequences provide a uniquethat may then be used for the efficient conversion of pentose sugars (xylose, arabinose) to ethanol. Method of using theintegrants and derivatives thereof in production of ethanol from cellulosic feedstock is also disclosed. The invention also provides a method for preparing aintegrant as part of the present invention. The hoststrain found particularly useful in the creation of these compositions and methods is31821.