The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 29, 2007
Filed:
Jun. 22, 2004
Andrew Robert Goodey, Mapperley Park, GB;
Darell Sleep, West Bridgford, GB;
Hendrik Van Urk, Radcliffe-on-Trent, GB;
Stephen Berezenko, Hucknall, GB;
John Rodney Woodrow, West Bridgford, GB;
Richard Alan Johnson, West Bridgford, GB;
Patricia Carol Wood, Burton-on-Trent, GB;
Stephen James Burton, Little Eversden, GB;
Alan Victor Quirk, Loughborough, GB;
Andrew Robert Goodey, Mapperley Park, GB;
Darell Sleep, West Bridgford, GB;
Hendrik Van Urk, Radcliffe-on-Trent, GB;
Stephen Berezenko, Hucknall, GB;
John Rodney Woodrow, West Bridgford, GB;
Richard Alan Johnson, West Bridgford, GB;
Patricia Carol Wood, Burton-on-Trent, GB;
Stephen James Burton, Little Eversden, GB;
Alan Victor Quirk, Loughborough, GB;
Novozymes Delta, Limited, Nottingham, GB;
Abstract
A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through positive mode cation exchange and then positive mode anion exchange chromatography. Other steps may also be employed, ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin by using blue dyes) and affinity chromatography binding contaminants by using an aminophenylboronic acid resin. Elution of albumin, with a compound having affinity for albumin, from a material having no specific affinity for albumin is also disclosed, as is removal of ammonium ions with a counter-ion.