The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 17, 2007
Filed:
Sep. 23, 2003
Steven Gutteridge, Wilmington, DE (US);
Timothy Caspar, Yorklyn, DE (US);
Daniel Cordova, Hockessin, DE (US);
Yong Tao, Newark, DE (US);
Lihong Wu, Newark, DE (US);
Rejane M. Smith, Elkton, MD (US);
Steven Gutteridge, Wilmington, DE (US);
Timothy Caspar, Yorklyn, DE (US);
Daniel Cordova, Hockessin, DE (US);
Yong Tao, Newark, DE (US);
Lihong Wu, Newark, DE (US);
Rejane M. Smith, Elkton, MD (US);
E.I. du Pont de Nemours and Company, Wilmington, DE (US);
Abstract
The genes encoding ryanodine receptor homologs have been characterized from multiple insect families including lepidopteran tobacco budworm (), homopteran green peach aphid (), corn plant hopper (), cotton melon aphid () and fruitfly (). The full-length genes have been isolated, cloned and amplified in bacterial cells. Expression in insect cells shows that the recombinant protein folds into a functional calcium release channel. The genes and their corresponding polypeptides have a number of uses including, but not limited to, the isolation of other pest ryanodine receptors, the development of screens to identify insecticidally active compounds, use of fragments of genes as pesticides, fragments of protein for antibody production, fragments of protein for determination of the structure of insecticide binding sites, and identification of insecticides that disrupt the calcium balance in cells through other messengers that interact with the receptor calcium release mechanism. Methods are outlined for overcoming toxic effects of expressing recombinant proteins in host cells.