The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 03, 2007

Filed:

Dec. 21, 2005
Applicants:

Robert Dicosimo, Chadds Ford, PA (US);

Mark S. Payne, Wilmington, DE (US);

Anna Panova, Hockessin, DE (US);

Jeffrey Thompson, Wilmington, DE (US);

Daniel P. O'keefe, Ridley Park, PA (US);

Inventors:

Robert DiCosimo, Chadds Ford, PA (US);

Mark S. Payne, Wilmington, DE (US);

Anna Panova, Hockessin, DE (US);

Jeffrey Thompson, Wilmington, DE (US);

Daniel P. O'Keefe, Ridley Park, PA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 7/42 (2006.01); C12P 7/40 (2006.01); C12N 9/80 (2006.01); C12N 15/74 (2006.01);
U.S. Cl.
CPC ...
Abstract

Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.


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