The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 03, 2007
Filed:
Jan. 13, 2004
Hiroyuki Ebinuma, Ryugasaki, JP;
Takami Sarashina, Naka-gun, JP;
Takuji Matsumoto, Ryugasaki, JP;
Masahiro Sekiguchi, Ryugasaki, JP;
Koji Ushizawa, Ryugasaki, JP;
Hiroyuki Ebinuma, Ryugasaki, JP;
Takami Sarashina, Naka-gun, JP;
Takuji Matsumoto, Ryugasaki, JP;
Masahiro Sekiguchi, Ryugasaki, JP;
Koji Ushizawa, Ryugasaki, JP;
Daiichi Pure Chemicals Co., Ltd., Tokyo, JP;
Abstract
The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.