The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 27, 2007

Filed:

Nov. 06, 2001
Applicants:

Takami Maekawa, Kawasaki, JP;

Maiko Mori, Kawasaki, JP;

Yoshiyuki Takahara, Kawasaki, JP;

Inventors:

Takami Maekawa, Kawasaki, JP;

Maiko Mori, Kawasaki, JP;

Yoshiyuki Takahara, Kawasaki, JP;

Assignee:

Ajinomoto Co., Inc., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); G01N 33/53 (2006.01); C12N 15/00 (2006.01); C07K 1/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

Each gene in a group comprising many genes is cloned into an expression vector so that its gene product should be expressed as a fusion protein with a protein emitting self-fluorescence to construct a gene expression library. The gene expression library is introduced into cells of two groups to express the fusion proteins in the cells, and only one cell group is stimulated by a drug or the like. Then, cells in which the fusion proteins are localized in the nuclei or their nuclei are isolated from cells of the two groups, respectively. By comparing these cells, genes coding for proteins that are transported into the nuclei specifically in response to a certain stimulus when the stimulus is applied to the cells are retrieved.


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