The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 20, 2007

Filed:

Apr. 09, 2003
Applicants:

Jay Keasling, Berkeley, CA (US);

Vincent Martin, Kensington, CA (US);

Douglas Pitera, Oakland, CA (US);

Sydnor T. Withers, Iii, Richmond, CA (US);

Jack Newman, Berkeley, CA (US);

Inventors:

Jay Keasling, Berkeley, CA (US);

Vincent Martin, Kensington, CA (US);

Douglas Pitera, Oakland, CA (US);

Sydnor T. Withers, III, Richmond, CA (US);

Jack Newman, Berkeley, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 7/42 (2006.01); C07H 21/04 (2006.01); C12N 9/00 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods for synthesizing amorpha-4,11-diene synthase from isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. Amorpha-4,11-diene synthase is then produced using an optimized amorpha-4,11-diene synthase gene. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.


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