The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 06, 2007
Filed:
Oct. 29, 2003
Douglas Nixon, San Francisco, CA (US);
Adrian B. Mcdermott, Leyburn, GB;
Scott Furlan, San Francisco, CA (US);
Martin Bigos, San Francisco, CA (US);
Megan Sheehy, Syracuse, NY (US);
Paul Klenerman, Oxford, GB;
Douglas Nixon, San Francisco, CA (US);
Adrian B. McDermott, Leyburn, GB;
Scott Furlan, San Francisco, CA (US);
Martin Bigos, San Francisco, CA (US);
Megan Sheehy, Syracuse, NY (US);
Paul Klenerman, Oxford, GB;
The J. David Gladstone Institutes, San Francisco, CA (US);
Abstract
The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.