The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 27, 2007
Filed:
Oct. 31, 2003
SE Ho Park, Daejon-Shi, KR;
Hae Sik Yang, Daejon-Shi, KR;
Dae Sik Lee, Daejon-Shi, KR;
Yong Beom Shin, Daejon-Shi, KR;
Kyu Won Kim, Daejon-Shi, KR;
Tae Hwan Yoon, Gyeonggi-Do, KR;
Sung Jin Kim, Daejon-Shi, KR;
Yun Tae Kim, Daejon-Shi, KR;
Se Ho Park, Daejon-Shi, KR;
Hae Sik Yang, Daejon-Shi, KR;
Dae Sik Lee, Daejon-Shi, KR;
Yong Beom Shin, Daejon-Shi, KR;
Kyu Won Kim, Daejon-Shi, KR;
Tae Hwan Yoon, Gyeonggi-Do, KR;
Sung Jin Kim, Daejon-Shi, KR;
Yun Tae Kim, Daejon-Shi, KR;
Electronics and Telecommunications Research Institute, Daejon-shi, KR;
Abstract
The present invention is directed to an improved multiplex PCR method for obtaining at least two PCR products from one PCR solution. In the multiplex PCR method for having at least two DNA amplified products from a sample positioned in a PCR equipment, the object of the present invention is to provide a novel multiplex PCR method characterized in that a primer annealing temperature and an extension time be changed per cycle with constant periods. When a multiplex PCR is performed in accordance with the present invention, limitations in determining PCR conditions due to a various sizes of PCR products or dimers caused by primers can be eliminated, and time and efforts required for determining the PCR conditions to various samples can also be reduced. Further, not only refined DNAs like cDNA, genomic DNA, vector, etc, but blood can be directly used as a multiplex PCR sample, and PCR amplification reaction can be performed even with a sample having the smallest amounts.