The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 13, 2007
Filed:
May. 15, 2001
Grigoriy S. Tchaga, Newark, CA (US);
George G. Jokhadze, Mountain View, CA (US);
Grigoriy S. Tchaga, Newark, CA (US);
George G. Jokhadze, Mountain View, CA (US);
Clontech Laboratories, Inc., Mountain View, CA (US);
Abstract
The present invention provides metal ion affinity peptides, fusion proteins comprising metal ion affinity peptides, and polynucleotides encoding the fusion proteins. A feature of the subject invention is that the metal ion affinity peptide has a formula selected from the group consisting of: formula 1: (His-X-X)-(His-X-X-X)-(His-X), wherein each of Xand Xis independently an amino acid with an aliphatic or an amide side chain, each of X, X, Xis independently an amino acid with a basic side chain (except His) or an acidic side chain, each Xis an amino acid with an aliphatic or an amide side chain, n1 and n2 are each independently 1–3, and n3 is 1–5; formula 2: (His-Asn), where n=3 to 10; and formula 3: (His-X-X), wherein each of Xand Xis an amino acid having an acidic side chain, and n=3 to 10. The invention further provides recombinant vectors comprising subject polynucleotides, and host cells comprising the recombinant vectors. The invention further provides methods and kits for purifying a fusion protein comprising a metal ion affinity peptide.