The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 30, 2007
Filed:
Jul. 06, 2001
Duarte Miguel DE Franca Teixeira Dos Prazeres, Lisbon, PT;
Maria Margarida Fonseca Rodrigues Diogo, Lisbon, PT;
Joao Antonio DE Sampaio Rodrigues Queiroz, Covilha, PT;
Duarte Miguel de Franca Teixeira dos Prazeres, Lisbon, PT;
Maria Margarida Fonseca Rodrigues Diogo, Lisbon, PT;
Joao Antonio De Sampaio Rodrigues Queiroz, Covilha, PT;
Instituto Superior Tecnico, Lisbon, PT;
Universidade da Beira Interior, Covilha, PT;
Abstract
The invention refers to a new process for the production of purification of high purity plasmid DNA. The process comprehends: a) the production of cells containing plasmid DNA, b) the disruption of the cells in order to obtain a lysate containing plasmid DNA, c) a concentration step by precipitation with isopropanol, d) a pre-purification and conditioning step by the addition of ammonium sulphate, e) a purification step using hydrophobic interaction chromatography, f) a final concentration and/or buffer exchange step. The process is scaleable, it does not use enzymes or mutagenic agents and it enables the preparation of plasmid DNA with pharmaceutical grade, which complies with the requirements of regulatory agencies. The invention belongs to the technical domain of Biochemical Engineering/Biotechnology.
Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile