The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 30, 2007
Filed:
Jan. 17, 2003
Christoph Eckerskorn, Ottobrunn, DE;
Hans-jörg Grill, Männedorf, CH;
Gerhard Weber, Kirchheim, DE;
Peter Weber, München, DE;
David Yost, Poolesville, MD (US);
Christoph Eckerskorn, Ottobrunn, DE;
Hans-Jörg Grill, Männedorf, CH;
Gerhard Weber, Kirchheim, DE;
Peter Weber, München, DE;
David Yost, Poolesville, MD (US);
Tecan Trading AG, Mannedorf, CH;
Abstract
A method for separating particles in free-flow electrophoresis FFE includes conveying a separation medium using a dosage pump into a separation chamber; releasing the separation medium via outlets; applying an electrical field in the separation medium; coupling a particle to be separated with selectively defined charge labels for generating charge-modified particles which due to their selectively modified net surface charge display a different migratory behavior in FFE than non-charge-modified particles; adding a mixture of particles to be separated to the separation medium in the separation chamber by way of sample injection points; removing the particles separated using the separation medium by way of fractionation points; and collecting the fractions with the separated particles. Focusing buffers are created using a buffer medium between the separation medium and at least one electrode, whereby this buffer medium is much more electrically conductive than the separation medium and is fed into these focusing buffers.