The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 23, 2007
Filed:
Dec. 20, 2002
Timothy K. Nadler, Framingham, MA (US);
Kenneth G. Parker, Hopkinton, MA (US);
George J. Vella, Medway, MA (US);
Barrie G. Wagenfeld, Saugus, MA (US);
Yulin Huang, Westwood, MA (US);
Robert J. Lotti, East Boston, MA (US);
Timothy K. Nadler, Framingham, MA (US);
Kenneth G. Parker, Hopkinton, MA (US);
George J. Vella, Medway, MA (US);
Barrie G. Wagenfeld, Saugus, MA (US);
Yulin Huang, Westwood, MA (US);
Robert J. Lotti, East Boston, MA (US);
PerSeptive Biosystems Inc., Framingham, MA (US);
Abstract
Polypeptides are electroblotted through a digestion membrane to a composite capture membrane that can be directly analyzed using mass spectrometry. The molecular weights of the fragments generated by the digestion membrane are then used to identify the polypeptide from which they originated. The digestion membrane contains an immobilized protease such as trypsin, which cleaves the electroblotted polypeptides into fragments during electroblotting with such high enzyme cleavage capacity and efficiency that one pass of the polypeptide through the membrane is sufficient. The peptide fragments are collected onto a composite capture membrane that is chemically treated, for example by adding a mixture of nitrocellulose and MALDI matrix, so as to absorb peptides near the surface to facilitate desportion, thereby increasing the sensitivity of subsequent analysis by MALDI-TOF mass spectrometry. A wide variety of application are disclosed including identifying proteins separated on a gel or within a tissue sample.