The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 16, 2007

Filed:

Aug. 05, 2004
Applicants:

J. Gregory Zeikus, Okemos, MI (US);

Maris Laivenieks, East Lansing, MI (US);

Claire Vieille, Lansing, MI (US);

Pil Kim, Seoul, KR;

Inventors:

J. Gregory Zeikus, Okemos, MI (US);

Maris Laivenieks, East Lansing, MI (US);

Claire Vieille, Lansing, MI (US);

Pil Kim, Seoul, KR;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/46 (2006.01); C12N 15/00 (2006.01); C12N 15/74 (2006.01);
U.S. Cl.
CPC ...
Abstract

Anplasmid vector which provides a means to overexpress proteins in. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner in. The plasmid comprises at least one marker gene, operably linked to a first promoter functional in, an origin of replication functional in, a second promoter isolated from, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence of, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinantis described, including a method of transformation. Additionally, a recombinantis disclosed and a method for producing succinate utilizing this recombinantis described.


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