The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 12, 2006
Filed:
Mar. 23, 2005
Alan L. Greener, San Diego, CA (US);
Lisa Joy Hexdall, San Diego, CA (US);
Carsten Peter Carstens, San Diego, CA (US);
Joseph A. Sorge, Del Mar, CA (US);
Alan L. Greener, San Diego, CA (US);
Lisa Joy Hexdall, San Diego, CA (US);
Carsten Peter Carstens, San Diego, CA (US);
Joseph A. Sorge, Del Mar, CA (US);
Stratagene California, La Jolla, CA (US);
Abstract
The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.