The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 12, 2006
Filed:
Oct. 30, 2000
Jean-pierre Perreault, Fleurimont, CA;
Sirinart Ananvoranich, Westbury, CA;
Daniel Lafontaine, Saint-Alexis de Montcalm, CA;
Jean-Pierre Perreault, Fleurimont, CA;
Sirinart Ananvoranich, Westbury, CA;
Daniel Lafontaine, Saint-Alexis de Montcalm, CA;
Universite de Sherbrooke, Quebec, CA;
Abstract
A method is described for cleaving a nucleic acid substrate with a nucleic acid enzyme at a cleavage site comprising mixing the substrate with the enzyme, wherein the substrate includes a 7 nucleotide sequence with at least 6 nucleotides 3' to the cleavage site and at least 1 nucleotide 5′ to the cleavage site and of formula:5′-H′GNNHNN-3′wherein each N is a nucleotide which may be the same or different, H is a nucleotide selected from the group consisting of A, U, C, and T, and is the site of cleavage, and H′ is a ribonucleotide selected from the group consisting of A, U, and C, wherein (i) the first nucleotide 3′ to the cleavage site is capable of forming a wobble pair with the enzyme, (ii) the second, third, fifth, and sixth nucleotides 3′ to the cleavage site are capable of forming conventional Watson-Crick base pairs with the enzyme, (iii) the fourth nucleotide 3′ to the cleavage site is capable of forming a non-conventional Watson-Crick base pair with the enzyme, and (iv) the first nucleotide 5′ to the cleavage site does not form a base pair with the enzyme; and the enzyme comprises a substrate binding portion which is capable of base pairing to the 6 nucleotides 3′ to the cleavage site of the substrate and which binding portion comprises the sequence:3′-UNNXNN-5′wherein each N is a nucleotide which may be the same or different, and X is a nucleotide selected from the group consisting of T, U, A, and G, whereby binding of the substrate to the enzyme effects cleavage of the substrate at the cleavage site.