The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 12, 2006

Filed:

May. 19, 2000
Applicants:

Frank P. Luyten, Rockville, MD (US);

Malcolm Moos, Jr., Bethesda, MD (US);

Steven Chao-huan Chang, Chicago, IL (US);

Inventors:

Frank P. Luyten, Rockville, MD (US);

Malcolm Moos, Jr., Bethesda, MD (US);

Steven Chao-Huan Chang, Chicago, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01); C07K 14/51 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention is a purified cartilage extract that stimulates local cartilage formation when combined with a matrix and implanted into a mammal. This extract can conveniently be produced by a method which includes the steps of: obtaining cartilage tissue; homogenizing the cartilage tissue in the presence of chaotropic agents under conditions that permit separation of proteins from proteoglycans; separating the proteins from the proteoglycans and then obtaining the proteins. The step for separating the proteins from the proteoglycans can be carried out using a sepharose column. The extract can also be obtained by additionally including the steps of separating the proteins on a molecular sieve and then collecting the proteins having molecular weights in the 30 kDa to 60 kDa size range. Articular cartilage or epiphyseal cartilage can be used in the preparation of this purified extract.


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